CES3 抗体 (HRP)
Quick Overview for CES3 抗体 (HRP) (ABIN7565877)
抗原
See all CES3 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Esterase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
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产品特性
- Synonyms: rabbit anti-Esterase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
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纯化方法
- Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
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anti-Carboxylesterase 3 (CES3) (AA 65-233) antibody
CES3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) antibodyCES3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (AA 1-568) antibodyCES3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (AA 269-568) antibodyCES3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (C-Term) antibodyCES3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Esterase Peroxidase conjugated antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:250 - 1:1,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CES3 (Carboxylesterase 3 (CES3))
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别名
- CES3
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背景
- Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.
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基因ID
- 100626873, 343791015
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UniProt
- Q29550
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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