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CELA1 抗体 (HRP)

This anti-CELA1 antibody is a 兔 多克隆 antibody detecting CELA1 in WB, IHC 和 ELISA. Suitable for Pig.
产品编号 ABIN7565876
发货至: 中国

Quick Overview for CELA1 抗体 (HRP) (ABIN7565876)

抗原

See all CELA1 抗体
CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

适用

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Pig

宿主

  • 32
  • 5

克隆类型

  • 33
  • 4
多克隆

标记

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This CELA1 antibody is conjugated to HRP

应用范围

  • 29
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • 原理

    Elastase Antibody Peroxidase Conjugated

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].

    产品特性

    Synonyms: rabbit anti-Elastase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

    纯化方法

    Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Elastase [Porcine Pancreas]

    Immunogen Type: Native Protein

  • 应用备注

    Application Note: Anti-Elastase Peroxidase conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Elastase [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原

    CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

    别名

    CELA1

    背景

    Background: Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. Elastase also has the important immunological role of breaking down Shigella virulence factors. Elastase is responsible for pulmonary emphysema, cyclic hematopoiesis, and blistering in bullous pemphigoid.

    基因ID

    396766

    NCBI登录号

    NP_998988

    UniProt

    P00772
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