CELA1 抗体 (HRP)
Quick Overview for CELA1 抗体 (HRP) (ABIN7565876)
抗原
See all CELA1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Elastase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].
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产品特性
- Synonyms: rabbit anti-Elastase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody
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纯化方法
- Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Elastase [Porcine Pancreas]
Immunogen Type: Native Protein
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anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 40-258) antibody
CELA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 159-257) antibodyCELA1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 44-259) antibodyCELA1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 1-258) antibodyCELA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 80-129) antibodyCELA1 适用: 人, 小鼠, Pig, 大鼠, Cow, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (N-Term) antibodyCELA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人 WB, EIA, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
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应用备注
- Application Note: Anti-Elastase Peroxidase conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Elastase [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))
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别名
- CELA1
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背景
- Background: Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. Elastase also has the important immunological role of breaking down Shigella virulence factors. Elastase is responsible for pulmonary emphysema, cyclic hematopoiesis, and blistering in bullous pemphigoid.
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基因ID
- 396766
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NCBI登录号
- NP_998988
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UniProt
- P00772
抗原
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