Catalase 抗体 (HRP)
Quick Overview for Catalase 抗体 (HRP) (ABIN7565869)
抗原
See all Catalase (CAT) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Catalase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine Liver].
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产品特性
- Synonyms: rabbit anti-Catalase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Catalase Antibody, Cas 1 antibody, CAT antibody, Cs1 antibody, MGC138422 antibody, MGC138424 antibody
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纯化方法
- Catalase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Catalase [Bovine Liver]
Immunogen Type: Native Protein
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anti-Catalase (CAT) (AA 401-523) antibody
CAT 适用: 人 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (Internal Region) antibodyVerified CAT 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Catalase (CAT) antibodyCAT 适用: 人, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Catalase (CAT) (AA 251-350) antibodyCAT 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 32-157) antibodyCAT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 1-527) antibodyCAT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 1-527) antibodyCAT 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Catalase (CAT) antibodyCAT 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (C-Term) antibodyCAT 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Catalase (CAT) (AA 80-239) antibodyCAT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Catalase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Catalase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500- 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Catalase (CAT)
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别名
- CAT
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背景
- Background: Catalase is found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species.
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基因ID
- 531682
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UniProt
- P00432
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途径
- Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
抗原
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