CPA1 抗体 (HRP)
Quick Overview for CPA1 抗体 (HRP) (ABIN7565867)
抗原
See all CPA1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Carboxypeptidase A Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-Carboxypeptidase A antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
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纯化方法
- Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein
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anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 205-419) antibody
CPA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) antibodyCPA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPA1-2714 unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) antibodyCPA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPA1-2711 unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) antibodyCPA1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) antibodyCPA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3F11 unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (Internal Region) antibodyCPA1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) antibodyCPA1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-601M unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 201-300) antibodyCPA1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 111-419) antibodyCPA1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A1 (Pancreatic) (CPA1) (AA 1-419) antibodyCPA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Carboxypeptidase A Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:300 - 1:2,000 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
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别名
- CPA1
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背景
- Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.
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基因ID
- 286762
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NCBI登录号
- NP_777175
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UniProt
- P00730
抗原
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