CA2 抗体 (HRP)
Quick Overview for CA2 抗体 (HRP) (ABIN7565865)
抗原
See all CA2 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Carbonic Anhydrase II Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
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产品特性
- Synonyms: rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II
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纯化方法
- Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]
Immunogen Type: Native Protein
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anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibody
CA2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibodyCA2 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (C-Term) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 54-83), (N-Term) antibodyCA2 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB18368 unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 201-260) antibodyCA2 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Carbonic anhydrase II Peroxidase Conjugated Antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CA2 (Carbonic Anhydrase II (CA2))
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别名
- CA2
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背景
- Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
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基因ID
- 280740
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NCBI登录号
- NP_848667
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UniProt
- P00921
抗原
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