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CA1 抗体 (HRP)

This anti-CA1 antibody is a 山羊 多克隆 antibody detecting CA1 in WB, ELISA 和 IHC. Suitable for 人.
产品编号 ABIN7565864
发货至: 中国

Quick Overview for CA1 抗体 (HRP) (ABIN7565864)

抗原

See all CA1 抗体
CA1 (Carbonic Anhydrase I (CA1))

适用

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  • 1
  • 1

宿主

  • 97
  • 10
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  • 1
  • 1
山羊

克隆类型

  • 94
  • 22
多克隆

标记

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  • 19
  • 8
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  • 3
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  • 1
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This CA1 antibody is conjugated to HRP

应用范围

  • 81
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  • 7
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 原理

    Carbonic Anhydrase I Antibody Peroxidase Conjugated

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ].

    产品特性

    Synonyms: goat anti-Carbonic Anhydrase I Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

    纯化方法

    Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Carbonic Anhydrase I [Human Erythrocytes ]

    Immunogen Type: Native Protein

  • 应用备注

    Application Note: Anti-Carbonic Anhydrase I peroxidase is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原

    CA1 (Carbonic Anhydrase I (CA1))

    别名

    CA1

    背景

    Background: Carbonic Anhydrase I is an enzyme that in humans is encoded by the CA1 gene. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.

    基因ID

    759

    NCBI登录号

    NP_001122301

    UniProt

    P00915
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