Serum 抗体 (HRP)
This 兔 多克隆 antibody specifically detects Serum in ELISA, WB, IHC 和 DB. It exhibits reactivity toward Cow.
产品编号 ABIN7565863
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Serum 抗体 (HRP) (ABIN7565863)
抗原
Serum
适用
Cow
宿主
兔
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
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原理
- Bovine Serum Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.
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产品特性
- Synonyms: Rabbit anti-Bovine Serum Peroxidase Conjugated Antibody, Rabbit anti-Bovine Serum Antibody HRP Conjugation
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纯化方法
- Bovine serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Bovine serum proteins
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Bovine Serum peroxidase conjugate has been tested by dot blot and is suitable to be assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized ELISA Dilution: User Optimized Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Serum
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背景
- Background: Anti-Bovine serum antibody detects bovine serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Bovine serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.
抗原
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