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beta-Amylase 1 抗体 (HRP)

This anti-beta-Amylase 1 antibody is a 兔 多克隆 antibody detecting beta-Amylase 1 in WB, ELISA 和 DB. Suitable for Sweet potato.
产品编号 ABIN7565858
发货至: 中国

Quick Overview for beta-Amylase 1 抗体 (HRP) (ABIN7565858)

抗原

beta-Amylase 1 (BAM1)

适用

  • 4
  • 1
Sweet potato

宿主

  • 5

克隆类型

  • 5
多克隆

标记

  • 3
  • 1
  • 1
This beta-Amylase 1 antibody is conjugated to HRP

应用范围

  • 5
  • 4
  • 2
  • 1
Western Blotting (WB), ELISA, Dot Blot (DB)
  • 原理

    Beta Amylase Antibody Peroxidase Conjugated

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato].

    产品特性

    Synonyms: rabbit anti-Beta Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

    纯化方法

    Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Beta Amylase [Sweet Potato]

    Immunogen Type: Native Protein

  • 应用备注

    Application Note: Anti-Beta Amylase Peroxidase conjugated antibody has been tested by ELISA and dot blot This product is assayed against 1.0 μg of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原

    beta-Amylase 1 (BAM1)

    别名

    BMY1, AMYB

    背景

    Background: Beta Amylase acts on starch, glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion.

    UniProt

    P10537
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