beta-Amylase 1 抗体 (HRP)
This anti-beta-Amylase 1 antibody is a 兔 多克隆 antibody detecting beta-Amylase 1 in WB, ELISA 和 DB. Suitable for Sweet potato.
Quick Overview for beta-Amylase 1 抗体 (HRP) (ABIN7565858)
抗原
beta-Amylase 1 (BAM1)
适用
Sweet potato
宿主
兔
克隆类型
多克隆
标记
This beta-Amylase 1 antibody is conjugated to HRP
应用范围
Western Blotting (WB), ELISA, Dot Blot (DB)
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原理
- Beta Amylase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Beta Amylase [Sweet Potato].
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产品特性
- Synonyms: rabbit anti-Beta Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody
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纯化方法
- Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Beta Amylase [Sweet Potato]
Immunogen Type: Native Protein
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anti-beta-Amylase 1 (BAM1) antibody (Biotin)
BAM1 适用: Sweet potato WB, ELISA, DB 宿主: 兔 Polyclonal Biotin
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应用备注
- Application Note: Anti-Beta Amylase Peroxidase conjugated antibody has been tested by ELISA and dot blot This product is assayed against 1.0 μg of Beta Amylase [Sweet Potato] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- beta-Amylase 1 (BAM1)
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别名
- BMY1, AMYB
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背景
- Background: Beta Amylase acts on starch, glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion.
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UniProt
- P10537
抗原
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