GOT1 抗体 (HRP)
Quick Overview for GOT1 抗体 (HRP) (ABIN7565857)
抗原
See all GOT1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- Aspartate Transaminase Antibody Peroxidase Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
-
产品特性
- Synonyms: sheep anti-Aspartate Transaminase HRP conjugated Antibody, peroxidase conjugated sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody
-
纯化方法
- Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein
-
-
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibody
GOT1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 人 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) antibodyGOT1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 244-413) antibodyGOT1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (N-Term) antibodyGOT1 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 山羊, 豚鼠, 马, 兔, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-411) antibodyGOT1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) antibodyGOT1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) antibodyGOT1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Application Note: Anti-Aspartate Transaminase Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
-
别名
- GOT1
-
背景
- Background: Aspartate Transaminase is an important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Aspartate Transaminase is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells, and it is commonly measured clinically as a marker for liver health.
-
基因ID
- 396967
-
NCBI登录号
- NP_999092
-
UniProt
- P00503
-
途径
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
抗原
-
