Liver Arginase 抗体 (HRP)
Quick Overview for Liver Arginase 抗体 (HRP) (ABIN7565855)
抗原
See all Liver Arginase (ARG1) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Arginase Antibody Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].
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产品特性
- Synonyms: rabbit anti-Arginase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Arginase Antibody, A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody
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纯化方法
- Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Arginase [Bovine Liver]
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Arginase Peroxidase Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Arginase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
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别名
- ARG1
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背景
- Background: Arginase is a manganese-containing enzyme belonging to the ureohydrolase family of enzymes. Arginase catalyzes the fifth and final step in the urea cycle, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea.
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基因ID
- 513608
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NCBI登录号
- NP_001039619
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UniProt
- Q2KJ64
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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