Uricase (ABC3735) 抗体 (HRP)
Quick Overview for Uricase (ABC3735) 抗体 (HRP) (ABIN7565853)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Uricase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].
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产品特性
- Synonyms: goat anti-uricase antibody, HRP conjugated goat anti-uricase antibody, Urate oxidase antibody
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纯化方法
- Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
Immunogen Type: Native Protein
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anti-Uricase (ABC3735) antibody (HRP)
ABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal HRP
anti-Uricase (ABC3735) antibody (Biotin)ABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal Biotin
anti-Uricase (ABC3735) antibodyABC3735 适用: Bacillus ELISA, IP, WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Uricase (ABC3735)
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别名
- ABC3735
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背景
- Background: Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localized in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form, and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
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基因ID
- 3202700
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NCBI登录号
- YP_177228
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UniProt
- Q5WBJ3
抗原
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