TUBA1B 抗体 (C-Term)
Quick Overview for TUBA1B 抗体 (C-Term) (ABIN7565852)
抗原
See all TUBA1B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Alpha-Tubulin Antibody
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交叉反应 （详细）
- This Loading Control antibody is directed against alpha tubulin.
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产品特性
- Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control
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纯化方法
- Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
- Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
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亚型
- IgG1 kappa
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anti-Tubulin, alpha 1B (TUBA1B) antibody
TUBA1B 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC, FM, MA 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 425-451) antibodyTUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, FM 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 29-310) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 437-441) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts. Immunohistochemistry Dilution: 2.5 μg/mL Western Blot Dilution: 1:1,000 ELISA Dilution: 1:300,000 IF Microscopy Dilution: 0.1 μg/mL Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- TUBA1B (Tubulin, alpha 1B (TUBA1B))
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别名
- TUBA1B
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背景
- Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
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基因ID
- 17986283
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UniProt
- P68363
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途径
- Microtubule Dynamics, M Phase
抗原
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