SOXA 抗体 (HRP)
SOXA
适用: Microbial
ELISA, IP, WB
宿主: 山羊
Polyclonal
HRP
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- 抗原 See all SOXA products
- SOXA (SoxA Gene Product (SOXA))
- 适用
- Microbial
- 宿主
- 山羊
- 克隆类型
- 多克隆
- 标记
- This SOXA antibody is conjugated to HRP
- 应用范围
- ELISA, Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Sarcosine Oxidase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].
- 产品特性
- Synonyms: goat anti-Sarcosine Oxidase Antibody, HRP Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit
- 纯化方法
- Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Sarcosine Oxidase [Microbial]
Immunogen Type: Native Protein
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- 应用备注
- Application Note: Anti-Sarcosine Oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Sarcosine Oxidase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- SOXA (SoxA Gene Product (SOXA))
- 别名
- soxA (SOXA 产品)
- 背景
- Background: Sarcosine oxidase is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide, and 5,10-methylenetetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.
- 基因ID
- 10586229
- NCBI登录号
- YP_004446819
- UniProt
- Q46337
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