Serum 抗体 (HRP)
The HRP-conjugated 兔 多克隆 anti-Serum antibody (ABIN7565832) specifically detects Serum in ELISA, WB, IHC 和 DB.
The antibody is reactive with 大鼠 samples.
产品编号 ABIN7565832
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Serum 抗体 (HRP) (ABIN7565832)
抗原
Serum
适用
大鼠
宿主
兔
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
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原理
- Rat Serum Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.
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产品特性
- Synonyms: rabbit anti-Rat Serum Antibody Peroxidase Conjugated, rabbit anti-Rat Serum HRP conjugated Antibody
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纯化方法
- Anti-Rat serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Rat Serum Proteins
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Rat serum Peroxidase Conjugated antibody has been tested by Dot blot and ELISA and is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized ELISA Dilution: User Optimized Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Serum
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背景
- Background: Anti-Rat serum antibody detects rat serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Rat serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.
抗原
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