Protein A 抗体 (Texas Red (TR))
Quick Overview for Protein A 抗体 (Texas Red (TR)) (ABIN7565827)
抗原
See all Protein A 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Protein A Antibody Texas Red™ Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum.
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产品特性
- Synonyms: goat anti-Protein A Antibody, Texas Red™ Conjugated goat anti-Protein A Antibody, Protein A Texas Red™, Texas Red™, Protein A Texas Red™ Antibody
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纯化方法
- Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Protein A [Staphylococcus aureus]
Immunogen Type: Native Protein
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Labeling Ratio
- 1.89
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anti-Protein A antibody
适用: Staphylococcus aureus ELISA, WB, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Rhodamine)适用: Staphylococcus aureus IF 宿主: 山羊 Polyclonal Rhodamine
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Protein A Texas Red Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Protein A
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背景
- Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.
抗原
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