Peroxidase 抗体 (Biotin)
Quick Overview for Peroxidase 抗体 (Biotin) (ABIN7565805)
抗原
See all Peroxidase 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Peroxidase (Horseradish) Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish].
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产品特性
- Synonyms: rabbit anti-Horseradish Peroxidase Antibody Biotin Conjugation, rabbit anti-HRP Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-HRP Antibody, Peroxidase Biotin Conjugated antibody, Anti-Peroxidase BAC Antibody
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纯化方法
- Anti-Peroxidase (Horseradish) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Peroxidase [Horseradish]
Immunogen Type: Native Protein
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anti-Peroxidase antibody
适用: Horseradish WB, ELISA, DB, IP 宿主: 山羊 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA, IHC 宿主: 豚鼠 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Peroxidase antibody适用: Horseradish WB, ELISA, IHC 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Peroxidase Antibody Biotin Conjugated has been tested by dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:250 Western Blot Dilution: 1:500 - 1:2,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Peroxidase
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背景
- Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Peroxidase antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.
抗原
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