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PAX6 抗体

This anti-PAX6 antibody is a 小鼠 单克隆 antibody detecting PAX6 in WB, ELISA 和 FM. Suitable for 人.
产品编号 ABIN7565804
发货至: 中国

Quick Overview for PAX6 抗体 (ABIN7565804)

抗原

See all PAX6 抗体
PAX6 (Paired Box 6 (PAX6))

适用

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宿主

  • 94
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小鼠

克隆类型

  • 80
  • 57
单克隆

标记

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This PAX6 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

克隆位点

7H10-D3-D4-G5
  • 原理

    PAX6 Antibody

    交叉反应 (详细)

    Cross-reactivity with PAX6 from other sources has not been determined.

    产品特性

    Synonyms: mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody

    纯化方法

    Anti-PAX6 was purified from clarified ascites by Protein A chromatography.

    过滤

    Sterile filtered

    免疫原

    Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.

    Immunogen Type: Recombinant Protein

    亚型

    IgG1 kappa
  • 应用备注

    Application Note: Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:75,000 - 1:150,000 IF Microscopy Dilution: 1:100

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原

    PAX6 (Paired Box 6 (PAX6))

    别名

    PAX6

    背景

    Background: Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

    基因ID

    5080

    NCBI登录号

    NP_000271

    UniProt

    P26367

    途径

    Peptide Hormone Metabolism, Carbohydrate Homeostasis
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