PAX7 抗体 (Internal Region)
Quick Overview for PAX7 抗体 (Internal Region) (ABIN7565803)
抗原
See all PAX7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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原理
- Pax7 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross reactivity with Pax7 protein from mouse (100 % homology) and human (66.
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产品特性
- Synonyms: rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7
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纯化方法
- Anti-Pax-7 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Immunogen Type: Conjugated Peptide
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应用备注
- Application Note: Anti-Pax7 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:10,000-1:40:000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PAX7 (Paired Box 7 (PAX7))
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别名
- Pax7
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背景
- Background: The Anti-Pax7 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Paired box protein Pax-7 transcription factor plays a role in myogenesis through regulation of muscle precursor cells proliferation. These genes play critical roles during fetal development and cancer growth. It is expressed specifically during the development of the nervous and muscular system. Anti-Pax7 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
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基因ID
- 18509
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NCBI登录号
- NP_035169
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UniProt
- P47239
抗原
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