LYZ 抗体 (HRP)
Quick Overview for LYZ 抗体 (HRP) (ABIN7565788)
抗原
See all LYZ 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Lysozyme Antibody Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White].
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产品特性
- Synonyms: rabbit anti-Lysozyme Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White
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纯化方法
- Anti-Lysozyme Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Lysozyme Peroxidase Conjugate Antibody has been tested by western blotting and ELISA and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- LYZ (Lysozyme (LYZ))
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别名
- LYZ
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背景
- Background: Anti-Lysozyme Antibody detects lysozyme. Lysozyme belongs to the glycoside hydrolases enzymes that damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk, and mucus. It is also present in cytoplasmic granules of the polymorphonuclear neutrophils. Large amounts of lysozyme can be found in egg white. Anti-Lysozyme Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
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基因ID
- 396218
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NCBI登录号
- NP_990612
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UniProt
- P00698
抗原
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