Luciferase 抗体 (FITC)
Quick Overview for Luciferase 抗体 (FITC) (ABIN7565786)
抗原
See all Luciferase 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Luciferase Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum and purified and partially purified Luciferase (Photinus pyralis (Firefly)).
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产品特性
- Synonyms: goat anti-Luciferase Antibody FITC Conjugation, Fluorescein Conjugated goat anti-Luciferase Antibody
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纯化方法
- Anti-Luciferase Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Luciferase (Photinus pyralis (Firefly))
Immunogen Type: Native Protein
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Labeling Ratio
- 5.6
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anti-Luciferase antibody
适用: Firefly ELISA, IF (p), IF (cc), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IHC, FM 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IP 宿主: 小鼠 Monoclonal 2D4 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Luciferase Conjugated Antibody has been tested by western blot and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: User Optimized Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Luciferase
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背景
- Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.
抗原
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