Lactate Dehydrogenase A 抗体 (HRP)
Quick Overview for Lactate Dehydrogenase A 抗体 (HRP) (ABIN7565783)
抗原
See all Lactate Dehydrogenase A (LDHA) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Lactate Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
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产品特性
- Synonyms: goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M
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纯化方法
- Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.
Immunogen Type: Native Protein
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anti-Lactate Dehydrogenase A (LDHA) antibody
LDHA 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) antibodyLDHA 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (Middle Region) antibodyLDHA 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, 豚鼠, 犬, 山羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 154-182) antibodyLDHA 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 204-232) antibodyLDHA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 5-323) antibodyLDHA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (C-Term) antibodyLDHA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:6,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:3,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Lactate Dehydrogenase A (LDHA)
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别名
- LDHA
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背景
- Background: Anti-Lactate Dehydrogenase Antibody detects Lactate Dehydrogenase. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes, each acting on either D-lactate or L-lactate. The other two are NAD(P)-dependent enzymes, each acting on either D-lactate or L-lactate. Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate. Anti-Lactate Dehydrogenase Antibody is ideal for investigators involved in Cell Signaling, Immunology, Neuroscience and Signal Transduction research.
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基因ID
- 100009107, 126050
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UniProt
- P13491
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途径
- Warburg Effect
抗原
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