HBG1 抗体 (N-Term)
Quick Overview for HBG1 抗体 (N-Term) (ABIN7565772)
抗原
See all HBG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Hemoglobin beta F Antibody
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交叉反应 （详细）
- This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform.
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产品特性
- Synonyms: mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)
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纯化方法
- Anti-HbF is purified from tissue culture supernatant by protein A purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.
Immunogen Type: Conjugated Peptide
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亚型
- IgG1 kappa
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anti-Hemoglobin, gamma A (HBG1) (AA 44-106) antibody
HBG1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Hemoglobin, gamma A (HBG1) (AA 2-147) antibodyHBG1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin, gamma A (HBG1) (AA 1-147) antibodyHBG1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Hemoglobin, gamma A (HBG1) antibody (Biotin)HBG1 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-Hemoglobin, gamma A (HBG1) antibody (FITC)HBG1 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
anti-Hemoglobin, gamma A (HBG1) antibody (HRP)HBG1 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-Hemoglobin, gamma A (HBG1) (AA 1-100) antibodyHBG1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin, gamma A (HBG1) (AA 56-85) antibodyHBG1 适用: 人 WB 宿主: 兔 Polyclonal RB33610 unconjugated
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应用备注
- Application Note: Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HBG1 (Hemoglobin, gamma A (HBG1))
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别名
- HBG1
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背景
- Background: HbF antibodies detect the hemoglobin gamma isoform subunit. Functional alternate hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 gamma subunits (alpha-2 gamma-2). Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. HbF levels are increased to as much as 5 % to 10 % in normal pregnancy. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Hemoglobin variants arise from mutations in the globin genes and sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. HbF antibody does not react other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.
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基因ID
- 3047
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NCBI登录号
- NP_000550
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UniProt
- P69891
抗原
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