HBd 抗体 (N-Term)
Quick Overview for HBd 抗体 (N-Term) (ABIN7565770)
抗原
See all HBd 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Hemoglobin beta A-2 Antibody
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交叉反应 （详细）
- This protein A purified mouse monoclonal antibody reacts specifically with human HbA-2 delta isoform.
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产品特性
- Synonyms: mouse anti-HbA-2 antibody, mouse anti-hemoglobin antibody, Hemoglobin Subunit Delta, Hemoglobin Delta Chain 4, Delta-Globin 4, HbD, HbA-2 Antibody, Sickle Cell Disease (SCD)
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纯化方法
- Anti-HbA-2 is purified from tissue culture supernatant by protein A purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb delta-subunit conjugated to KLH.
Immunogen Type: Conjugated Peptide
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亚型
- IgG1 kappa
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anti-Hemoglobin, delta (HBd) (AA 1-143) antibody
HBd 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin, delta (HBd) (AA 2-147) antibodyHBd 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin, delta (HBd) (AA 2-147) antibodyHBd 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin, delta (HBd) (AA 1-147) antibodyHBd 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Hemoglobin beta A-2 (MOUSE) antibody has been tested by ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.00 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HBd (Hemoglobin, delta (HBd))
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别名
- HBD
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背景
- Background: HbA-2 or hemoglobin delta subunit antibodies detect the delta-specific sequence in the hemoglobin delta-subunit found in HbA-2. Functional hemoglobin (Hb) is a hetero tetramer and the dominant form of Adult Hb is composed of 2 alpha and 2 beta subunits (α2β2). Hemoglobin A-2 (HbA-2) is a normal but minor variant of hemoglobin A that consists of two alpha and two delta chains (α2δ2). Hemoglobin A-2 may be increased in beta thalassemia or in people who are heterozygous for the beta thalassemia gene, and HbA2 is also linked to neurological disorders. HbA-2 form exists in small amounts in all adult humans (1.5-3.1 % of all hemoglobin molecules) and is increased in people with Sickle-cell disease. Its normal biological role is not well understood. HbA-2 antibody does not react other forms of Hb including no cross-reaction to HbA or beta subunit. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.
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基因ID
- 3045
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NCBI登录号
- NP_000510
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UniProt
- P02042
抗原
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