HAO1 抗体 (HRP)
Quick Overview for HAO1 抗体 (HRP) (ABIN7565768)
抗原
See all HAO1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Glucose Oxidase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
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产品特性
- Synonyms: sheep anti-Glucose Oxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
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纯化方法
- Anti-Glucose Oxidase (Sheep) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
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anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 113-369) antibody
HAO1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) antibodyHAO1 适用: 小鼠, 大鼠 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 3B2 unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) antibodyHAO1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 161-273) antibodyHAO1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) antibodyHAO1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) antibodyHAO1 适用: 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal Mix unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) antibodyHAO1 适用: 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) antibodyHAO1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 251-300) antibodyHAO1 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, 豚鼠, 马, 猴, 兔, Bat, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) antibodyHAO1 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Glucose Oxidase peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: User Optimized Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))
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别名
- gox
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背景
- Background: Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Glucose Oxidase (Sheep) antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.
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UniProt
- P13006
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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