Avidin 抗体 (Texas Red (TR))
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- 抗原 See all Avidin (AVD) 抗体
- Avidin (AVD)
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Avidin antibody is conjugated to Texas Red (TR)
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应用范围
- Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 原理
- Avidin Antibody Texas Red™ Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Avidin.
- 产品特性
- Synonyms: rabbit anti-Avidin Antibody Texas Red™ Conjugation, Texas Red™ conjugated rabbit anti-Avidin antibody, Avidin Texas Red™, Anti-Avidin Texas Red™ Antibody, Egg White
- 纯化方法
- Anti-AVIDIN Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Avidin (Hen Egg White)
Immunogen Type: Native Protein
- Labeling Ratio
- 3.6
- Top Product
- Discover our top product AVD Primary Antibody
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anti-Avidin (AVD) antibody (Cy3)
AVD 适用: Chicken egg IHC (fro), IHC (p), ICC 宿主: Chicken eggs Polyclonal Cy3
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, DB, IP 宿主: 兔 Polyclonal unconjugated
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Application Note: Rabbit Anti-Avidin Antibody Texas Red Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: >1:1,000 FLISA Dilution: >1:2,000 IF Microscopy Dilution: User Optimized Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Avidin (AVD)
- 别名
- AVIDIN (AVD 产品)
- 背景
- Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30 % homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.
- UniProt
- P02701
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