Avidin 抗体 (HRP)
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- 抗原 See all Avidin (AVD) 抗体
- Avidin (AVD)
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Avidin antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
- 原理
- Avidin Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ].
- 产品特性
- Synonyms: rabbit anti-Avidin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Avidin antibody, Avidin Peroxidase, Anti-Avidin HRP Antibody, egg white
- 纯化方法
- Anti-Avidin Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Avidin (Hen Egg White)
Immunogen Type: Native Protein
- Top Product
- Discover our top product AVD Primary Antibody
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anti-Avidin (AVD) antibody (Cy3)
AVD 适用: Chicken egg IHC (fro), IHC (p), ICC 宿主: Chicken eggs Polyclonal Cy3
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, DB, IP 宿主: 兔 Polyclonal unconjugated
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Application Note: Anti-Avidin Antibody Peroxidase Conjugated has been tested in WB and assayed against 1.0 μg of Avidin [Hen Egg White ] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:27,000 of the reconstitution concentration is suggested for this product. This product can be suitable for ELISA, Western Blot, and Immunohistochemistry. Immunohistochemistry Dilution: 1:250 - 1:1,000 Western Blot Dilution: 1:1,000 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Avidin (AVD)
- 别名
- AVIDIN (AVD 产品)
- 背景
- Background: Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Avidin Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.
- UniProt
- P02701
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