Avidin 抗体 (HRP)
Quick Overview for Avidin 抗体 (HRP) (ABIN7565744)
抗原
See all Avidin (AVD) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Avidin Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ].
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产品特性
- Synonyms: rabbit anti-Avidin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Avidin antibody, Avidin Peroxidase, Anti-Avidin HRP Antibody, egg white
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纯化方法
- Anti-Avidin Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Avidin (Hen Egg White)
Immunogen Type: Native Protein
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anti-Avidin (AVD) antibody (Cy3)
AVD 适用: Chicken egg IHC (fro), IHC (p), ICC 宿主: Chicken eggs Polyclonal Cy3
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, DB, IP 宿主: 兔 Polyclonal unconjugated
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Avidin Antibody Peroxidase Conjugated has been tested in WB and assayed against 1.0 μg of Avidin [Hen Egg White ] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:27,000 of the reconstitution concentration is suggested for this product. This product can be suitable for ELISA, Western Blot, and Immunohistochemistry. Immunohistochemistry Dilution: 1:250 - 1:1,000 Western Blot Dilution: 1:1,000 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Avidin (AVD)
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别名
- AVIDIN
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背景
- Background: Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Avidin Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.
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UniProt
- P02701
抗原
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