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Avidin 抗体 (Biotin)

AVD 适用: 小鸡 WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
产品编号 ABIN7565741
发货至: 中国
  • 抗原 See all Avidin (AVD) 抗体
    Avidin (AVD)
    适用
    • 12
    • 7
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    小鸡
    宿主
    • 16
    • 8
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    克隆类型
    • 18
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    多克隆
    标记
    • 6
    • 5
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    This Avidin antibody is conjugated to Biotin
    应用范围
    • 21
    • 18
    • 16
    • 8
    • 6
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    原理
    Avidin Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified and partially purified Avidin [Hen Egg White].
    产品特性
    Synonyms: rabbit anti-Avidin Antibody biotin Conjugation, Biotin conjugated rabbit anti-Avidin antibody, Avidin BAC, Anti-Avidin biotin Antibody, Egg White
    纯化方法
    Anti-Avidin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Avidin (Hen Egg White)

    Immunogen Type: Native Protein

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  • 应用备注
    Application Note: Anti-Avidin Antibody Biotin Conjugated has been tested by ELISA and western blot and is suitable for immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:250 - 1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原
    Avidin (AVD)
    别名
    AVIDIN (AVD 产品)
    背景
    Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Avidin antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.
    UniProt
    P02701
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