AnsB 抗体 (HRP)
Quick Overview for AnsB 抗体 (HRP) (ABIN7565739)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- L-Asparaginase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E.
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产品特性
- Synonyms: rabbit anti-L-Asparaginase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
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纯化方法
- Anti-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Asparaginase Antibody was produced by repeated immunizations with E. coli Asparaginase protein.
Immunogen Type: Native Protein
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anti-AnsB antibody (Biotin)
适用: E. coli ELISA, WB, DB 宿主: 兔 Polyclonal Biotin
anti-AnsB antibody (HRP)适用: E. coli ELISA, WB 宿主: 兔 Polyclonal HRP
anti-AnsB antibody适用: E. coli ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Asparaginase Peroxidase conjugated antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and by ELISA. Researchers should determine optimal titers for applications that are not stated below. Western Blot Dilution: 1:200 - 1:1,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- AnsB
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背景
- Background: Anti-Asparaginase antibody detects Asparaginase. Asparaginase is an enzyme that catalyzes the hydrolysis of asparagine to aspartic acid. Asparaginases are naturally occurring enzymes expressed and produced by microorganisms. Anti-Asparaginase Antibody is ideal for investigators involved in Immunology, Cell Signaling, Neuroscience and Signal Transduction research.
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基因ID
- 1039460
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UniProt
- P00805
抗原
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