ACSS2 抗体 (N-Term)
Quick Overview for ACSS2 抗体 (N-Term) (ABIN7565732)
抗原
See all ACSS2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- ACSS2 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human ACSS2 protein.
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产品特性
- Synonyms: rabbit anti-ACSS2 antibody, ACSS-2, ACSS 2, Acetyl-coenzyme A synthetase (cytoplasmic), Acetate-CoA ligase, Acetyl-CoA synthetase, ACS, AceCS, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, ACAS2
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纯化方法
- Anti-ACSS2 Antibody to was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-terminus of human ACSS2.
Immunogen Type: Conjugated Peptide
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应用备注
- Application Note: Anti-ACSS2 Antibody has been tested in Western Blot and ELISA. Expect a band at approximately 76 kDa in western blot using appropriate lysates. Positive control whole cell lysates used Hep-G2 at 1 μg/mL for WB. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:10,000 - 1:50,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.06 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))
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别名
- ACSS2
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). ACSS2 (Acyl-CoA synthetase short-chain family member 2) is a protein coding gene encoding a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. This protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. This protein is upregulated in many cancers and is important for pro-survival under hypoxic conditions. ACSS2 may be associated with disorders such as Cystoisosporiasis. Anti-ACSS2 Antibody is useful for researchers interested in parasites, intestines, Glucose Energy Metabolism, and AKT research.
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基因ID
- 55902
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NCBI登录号
- NP_001070020
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UniProt
- Q9NR19
抗原
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