PhoA 抗体 (HRP)
The HRP-conjugated 兔 多克隆 anti-PhoA antibody (ABIN7565727) specifically detects PhoA in ELISA 和 WB.
The antibody is reactive with E. coli samples.
产品编号 ABIN7565727
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for PhoA 抗体 (HRP) (ABIN7565727)
抗原
PhoA
适用
E. coli
宿主
兔
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB)
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原理
- Alkaline Phosphatase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.
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产品特性
- Synonyms: rabbit anti-Alkaline Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
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纯化方法
- Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Alkaline Phosphatase [E.coli]
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Alkaline phosphatase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:15,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:4,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PhoA
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背景
- Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
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基因ID
- 945041
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NCBI登录号
- NP_414917
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UniProt
- P00634
抗原
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