ASRGL1 抗体 (HRP)
Quick Overview for ASRGL1 抗体 (HRP) (ABIN7565725)
抗原
See all ASRGL1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Alkaline Phosphatase Antibody HRP Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
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产品特性
- Synonyms: goat anti-Alkaline Phosphatase Antibody HRP Conjugation, Peroxidase Conjugation goat anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
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纯化方法
- Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein
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anti-Asparaginase Like 1 (ASRGL1) antibody
ASRGL1 适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal CRASH-1289 unconjugated
anti-Asparaginase Like 1 (ASRGL1) (Internal Region) antibodyVerified ASRGL1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Asparaginase Like 1 (ASRGL1) (AA 1-308) antibodyASRGL1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Asparaginase Like 1 (ASRGL1) (AA 1-180) antibodyASRGL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C8 unconjugated
anti-Asparaginase Like 1 (ASRGL1) (Internal Region) antibodyASRGL1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Asparaginase Like 1 (ASRGL1) antibodyASRGL1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal CRASH-1289 unconjugated
anti-Asparaginase Like 1 (ASRGL1) (AA 1-308) antibodyASRGL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Asparaginase Like 1 (ASRGL1) (AA 251-300) antibodyASRGL1 适用: 人, 小鼠, 豚鼠, 马, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Asparaginase Like 1 (ASRGL1) (C-Term) antibodyASRGL1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Asparaginase Like 1 (ASRGL1) (C-Term) antibodyASRGL1 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Alkaline Phosphatase peroxidase has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ASRGL1 (Asparaginase Like 1 (ASRGL1))
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别名
- ALP1
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背景
- Background: Alkaline Phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. Humans and most other mammals contain the following alkaline phosphatase isozymes: ALPI - intestinal, ALPL - tissue non-specific (liver/bone/kidney), ALPP - placental (Regan isozyme). Elevated ALP indicates that there could be active bone formation occurring as ALP is a byproduct of osteoblast activity (such as the case in Paget's disease of bone). Levels are also elevated in people with untreated Coeliac Disease. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
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基因ID
- 280993
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UniProt
- P19111
抗原
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