ADH1 抗体 (HRP)
Quick Overview for ADH1 抗体 (HRP) (ABIN7565722)
抗原
See all ADH1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Alcohol Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].
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产品特性
- Synonyms: rabbit anti-Alcohol Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC
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纯化方法
- Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Alcohol Dehydrogenase [Yeast]
Immunogen Type: Native Protein
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anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) (AA 169-370) antibody
ADH1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) (AA 25-228) antibodyADH1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibody (HRP)ADH1 适用: Saccharomyces cerevisiae WB, IHC, IP, ELISA 宿主: 兔 Polyclonal HRP
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibody (Biotin)ADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal Biotin
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Alcohol Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable in immunohistochemistry. Optimal titers are to be optimized by researchers. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:4,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
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别名
- ADH1
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背景
- Background: Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.
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基因ID
- 2538902, 12643994
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UniProt
- P00330
抗原
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