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IDE 抗体 (AA 491-590)

Name: IDE 抗体 (AA 491-590)
SKU: ABIN723680
Availability: OutOfStock
Rating: 5 (2 reviews)

This anti-IDE antibody is a 兔多克隆 antibody detecting IDE in WB, ELISA, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. Independently validated for use in Immunohistochemistry, Western Blotting.

Immunohistochemistry validation image for anti-Insulin-Degrading Enzyme (IDE) (AA 491-590) antibody (ABIN723680) (IDE 抗体 (AA 491-590))

IHC

Western Blotting validation image for anti-Insulin-Degrading Enzyme (IDE) (AA 491-590) antibody (ABIN723680) (IDE 抗体 (AA 491-590))

Formalin-fixed and paraffin-embedded human gastric carcinoma labeled with Rabbit Anti-IDE Polyclonal Antibody, Unconjugated (ABIN723680) at 1:300 followed by conjugation to the secondary antibody and DAB staining (IDE 抗体 (AA 491-590))

IHC

Formalin-fixed and paraffin-embedded mouse tumor labeled with Rabbit Anti-IDE Polyclonal Antibody, Unconjugated (ABIN723680) at 1:300 followed by conjugation to the secondary antibody and DAB staining (IDE 抗体 (AA 491-590))

8 images

(2 validations)

产品编号 ABIN723680

发货至: 中国

Datasheet as PDF

Quick Overview for IDE 抗体 (AA 491-590) (ABIN723680)

抗原

See all IDE 抗体

IDE (Insulin-Degrading Enzyme (IDE))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This IDE antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
15

8

8

5

5

3

3

2

2

2

1

1

1

1

1
AA 491-590

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Pig,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human IDE

亚型

IgG
anti-Insulin-Degrading Enzyme (IDE) (AA 753-973) antibody
IDE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) (AA 491-590) antibody
IDE 适用: 小鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 小鼠 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) (AA 1-250) antibody
IDE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) antibody
IDE 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 1H4 unconjugated

anti-Insulin-Degrading Enzyme (IDE) (AA 920-1019) antibody
IDE 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) (Internal Region) antibody
Verified IDE 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) (N-Term) antibody
IDE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) antibody
IDE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) (AA 501-800) antibody
IDE 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin-Degrading Enzyme (IDE) antibody
IDE 适用: 人 WB 宿主: 小鼠 Monoclonal 3H4 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #104435 (Immunohistochemistry)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#104435

日期

2023.03.15

抗原

IDE

Lot Number

9C07M588

Method validated

Immunohistochemistry

Positive Control

Adult mouse liver fixed in 4% paraformaldehyde

Negative Control

One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.

Notes

Passed. The IDE antibody (AA 491-590) ABIN723680 works in IHC-P at 1:100 concentrations with Tyramide amplification.

Validation Images

Staining of IDE positive cells in the adult mouse liver with ABIN723680.
Full Methods
Primary Antibody

ABIN723680

Secondary Antibody

poly-HRP conjugated goat anti-rabbit antibody

Full Protocol

Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
Wash, dehydrate, and embed samples in paraffin wax.
Wash several times with 0.01 M PBS.
Cut liver with a microtome into 20 µm sections and mount on glass slides.
After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
Incubate sections with primary rabbit anti-IDE (antibodies-online, ABIN723680, lot 9C07M588) diluted 1:50, 1:100, 1:200, and 1:300 in PBS-BSA-PLL ON at RT in a humid chamber.
Wash sections 3x 5 min with 0.01 M PBS.
Incubate sections with secondary poly-HRP conjugated goat anti-rabbit antibody from Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG (Thermo Fisher Scientific, B40922, lot 2465062) for 1 h at RT.
Wash sections 3x 5 min with 0.01 M PBS.
Incubate sections with Tyramide working solution containing 100X Tyramide stock solution (Alexa 488), 100X H₂O₂ solution and 1X Reaction buffer for 10 min.
Stop the reaction with the Reaction Stop Reagent working solution.
Wash sections 3x 5 min with 0.01M PBS.
Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.

Experimental Notes
生效 #104497 (Western Blotting)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#104497

日期

2023.03.15

抗原

IDE

Lot Number

9C07M588

Method validated

Western Blotting

Positive Control

Adult mouse brain, cerebellum, and liver

Negative Control

Notes

Passed. The IDE antibody (AA 491-590) ABIN723680 works in WB at 1:1000 concentrations with sensitive ECL substrate.

Validation Images

Western blot detection of IDE (110 kDa) in adult mouse brain (1), cerebellum (2), and liver (3) tissue homogenates with ABIN723680.
Full Methods
Primary Antibody

ABIN723680

Secondary Antibody

HRP-conjugated mouse anti-rabbit

Full Protocol

Homogenize tissues with cold lysis buffer containing 50 mM Tris HCl, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, and 1% protease inhibitor (Sigma P8340) using an ultrasonic homogenizer (MSE, SoniPrep 150) with 16 amplitude, 20 s on, 10 s off pulse for 60 s.
Centrifuge tissue homogentates at 13,000 rpm for 20 min at 4 °C.
Collect supernatants and Determine total protein content using a Bradford assay.
Denature 50 µg of total protein for 5 min at 90 °C and subsequently separate them on a denaturing 12% PAGE-SDS gel alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, 160374).
Electro-transfer proteins onto nitrocellulose membrane (Amerscham Biosciences, RPN203D) ON in the cold room.
Wash membrane 3x for 10 mon with 0.01 M PBS containing 0.1% Tween-20 (PBST).
Block membrane with PBST containing 2% bovine serum albumin for 1 h at RT.
Incubate membrane with primary rabbit anti-IDE antibody (antibodies-online, ABIN723680, lot 9C07M588) diluted 1:1,000 in PBST ON at 4 °C.
Wash membrane 3x 10 min with PBST.
Incubate membrane with secondary HRP-conjugated mouse anti-rabbit IgG (Sigma, A1949) diluted 1:4,000 in PBST for 1 h at RT.
Wash membrane 3x 10 min with PBST.
Visualize proteins with WesternBright Sirius HRP substrate (Advansta, K-12043) using a ChemiDoc Imaging System.

Experimental Notes
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

IDE (Insulin-Degrading Enzyme (IDE))

别名

IDE

背景

Synonyms: INSULYSIN, Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, IDE
Background: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

基因ID

3416

UniProt

P14735

途径

SARS-CoV-2 Protein Interactome