Recombinant Iba1 抗体
Quick Overview for Recombinant Iba1 抗体 (ABIN7543454)
抗原
See all Iba1 (IBA1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-AIF1 [GT1-mAb1], Rabbit IgG, lambda
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特异性
- This antibody is specific to < a href="https://everestbiotech.com/product/ebp05419-immunizing-peptide/" target="_blank">EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the < a href="https://everestbiotech.com/product/goat-anti-aif1-iba1-isoform-1-and-3-antibody/" target="_blank">polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.
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产品特性
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Original Species of Ab: Goat
Original Format of Ab: IgG
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.
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亚型
- IgG lambda
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anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
IBA1 适用: 人 WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibodyIBA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibodyIBA1 适用: 人 WB, IHC, IP, ICC, IF, IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibodyIBA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibodyIBA1 适用: 大鼠 WB 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibodyVerified IBA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibodyVerified IBA1 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibodyIBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibodyIBA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-146) antibodyIBA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AIF1-1909 unconjugated
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应用备注
- This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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别名
- AIF1
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背景
- AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1
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UniProt
- P55008
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途径
- Smooth Muscle Cell Migration
抗原
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