Recombinant HLA-DR10 抗体
The 兔 Chimeric anti-HLA-DR10 antibody (Clone Lym-1) (ABIN7543426) specifically detects HLA-DR10 in IHC, WB, FACS, IF, IP, RIA 和 StM.
The antibody is reactive with 人 samples.
产品编号 ABIN7543426
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for Recombinant HLA-DR10 抗体 (ABIN7543426)
抗原
HLA-DR10
抗体类型
Recombinant Antibody
适用
人
宿主
兔
克隆类型
Chimeric
标记
非结合性
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Staining Methods (StM)
克隆位点
Lym-1
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原理
- Anti-HLA-DR10 [Lym-1], Rabbit IgG, kappa
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特异性
- This antibody has high affinity for a discontinuous epitope on the β subunit of the human HLA-DR10 surface membrane antigen of malignant B cells, which is not expressed on normal lymphocytes. HLA-DR10 (DR10) is a HLA-DR serotype that recognizes the DRB1*1001 gene product.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing mice with tumor cell nuclei preparations as immunogens.
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亚型
- IgG kappa
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应用备注
- This antibody could recognize cell lines of B-cell lineage but not cells of T-cell, myeloid, or erythroid derivation in a live cell radioimmunoassay. Flow cytometric analysis of 22 lymphoma biopsies showed that the majority of B-cell tumors were LYM-1 positive. Approximately 40 % of B-cell chronic lymphocytic leukemias were found to be positive with Lym-1. The binding of this antibody to peripheral blot lymphocytes was assessed using indirect immunofluorescence staining. Immunoperoxidase staining of nonlymphoid human organs and tissues revealed weak reactivity of Lym-1. This antibody was also capable of immunoprecipitating HLA-DR10 antigen from Raji cells and ARH-77 myeloma cells. (PMID: 3542194). This antibody also was used for immunohistochemical staining of advanced B-cell NHL tissues from patients (PMID: 10809213). This antibody can be used to identify the HLA-DR variant HLA-DR10 in a western blot (PMID: 8917632). LYM-1 chimeric antigen receptor T cells were demonstrated to exhibit potent anti-tumor effects against B-cell lymphoma (PMID: 29261129).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- HLA-DR10
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背景
- CD74, Beta chain HLA-DR, HLA-DR β-chain, β-chain, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33
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UniProt
- P04233
抗原
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