Recombinant Laminin 抗体
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Quick Overview for Recombinant Laminin 抗体 (ABIN7543382)
抗原
See all Laminin (LAMA2) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Laminin-2 LG5 [C3_Hu10], Rabbit IgG, kappa
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特异性
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This antibody recognizes human and mouse laminin-2 LG5 and LG4/5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Application Notes original Clone: The binding specificity of this antibody for human and mouse laminin-2 LG5 was confirmed using ELISA. This antibody is capable of detecting human and mouse LG-4/5 expressed on HEK293 in a flow cytometry. This antibody can also be used for the identification recombinant human laminin-2 (Hu 211), murine LG-5 (mLG5), human LG-5 (hLG5), and human LG-4/5 (hLG4/LG5) in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody AS30_Hu6 to generate a duobody called TBTI (US11254737).
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交叉反应
- 小鼠
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.
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亚型
- IgG, kappa
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anti-Laminin, alpha 2 (LAMA2) (AA 3013-3122) antibody
LAMA2 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 2D4 unconjugated
anti-Laminin, alpha 2 (LAMA2) (AA 2051-2200) antibodyLAMA2 适用: 人, 小鼠, 大鼠, 马, Cow ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Laminin, alpha 2 (LAMA2) (AA 2901-3106) antibodyLAMA2 适用: 小鼠 IHC, WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Laminin, alpha 2 (LAMA2) (AA 974-1162) antibodyLAMA2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Laminin, alpha 2 (LAMA2) antibodyLAMA2 适用: 人 IHC, IHC (fro) 宿主: 小鼠 Monoclonal Mer3-22B2 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Human
OriginalFormat: IgG1
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))
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别名
- Laminin-2 LG5
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背景
- Merosin, Laminin subunit alpha-2, LAMA2, Laminin 211, laminin G-like 5 domain, LG5, g-like 4 and 5 domain, LG-4/5, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, C3, humanized C3
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UniProt
- P24043, Q60675
抗原
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