Recombinant F11R 抗体
Quick Overview for Recombinant F11R 抗体 (ABIN7543378)
抗原
See all F11R 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-JAM-A [6F4], Rabbit IgG, kappa
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特异性
- This antibody specifically recognizes human junctional adhesion molecule A and does not cross-react with other members of the JAM family. JAM-A is an adherens and tight junction protein expressed by endothelial and epithelial cells. It is recently identified as an antigenic protein (Ag) overexpressed in tumor cells and associated with cancer progression.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/c mice with MCF-7 cells. The humanized version was generated by grafting CDRs of parental mouse antibody onto human framework regions.
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亚型
- IgG kappa
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anti-F11 Receptor (F11R) (AA 1-299) antibody
F11R 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 2E3-1C8 unconjugated
anti-F11 Receptor (F11R) (AA 30-238) antibodyF11R 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) (pTyr280) antibodyF11R 适用: 人 WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) (AA 1-299) antibodyF11R 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) (Internal Region) antibodyF11R 适用: 人, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) (AA 64-249) antibodyF11R 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) antibodyF11R 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) (AA 31-224) antibodyF11R 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-F11 Receptor (F11R) antibodyF11R 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal H202-106 unconjugated
anti-F11 Receptor (F11R) (AA 1-299) antibodyF11R 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- This antibody was used for the characterization of human junctional adhesion molecule-A (JAM-A) and study of its expression on various cancerous tissues. The binding specificity of this antibody for human JAM-A expressed on CHO cells was confirmed using flow cytometry. JAM-A expression level on cell surface was determined by immunofluorescence staining and visualized by confocal microscopy. This antibody can also be used in the determination of JAM-A protein using western blot. Immunohistochemical analysis using this antibody revealed that JAM-A is overexpressed on breast, lung and kidney tumor tissues. Injections of anti-JAM-A antibody resulted in a significant tumor growth inhibition of xenograft human tumors in vivo. Treatment with monoclonal antibody induced a decrease of the Ki67 expression and downregulated JAM-A levels. This antigen may play a role in interfering with tumor proliferation (PMID: 22886345). This antibody binds the extracellular domain of JAM-A in an ELISA (Goetsch et al, 2011).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- F11R (F11 Receptor (F11R))
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别名
- JAM-A
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背景
- CD321, JAM-1, JAM1, JCAM, F11R, PAM-1, Junctional adhesion molecule A, Junctional adhesion molecule 1, Platelet F11 receptor
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UniProt
- Q9Y624
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途径
- Cell-Cell Junction Organization
抗原
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