Recombinant Lipoprotein Lipase 抗体
Quick Overview for Recombinant Lipoprotein Lipase 抗体 (ABIN7543369)
抗原
See all Lipoprotein Lipase (LPL) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Lipoprotein lipase [5D2], Mouse IgG1, kappa
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特异性
- The antibody is specific for lipoprotein lipase (LPL) and it binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The antibody does not cross react with mouse LPL. LPL is a key enzyme in triglyceride metabolism, it catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage.
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交叉反应
- Cat, 小鸡, Cow, 豚鼠, 大鼠
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing mice with bovine LPL.
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亚型
- IgG1 kappa
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anti-Lipoprotein Lipase (LPL) (AA 28-474) antibody
LPL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lipoprotein Lipase (LPL) (AA 300-327) antibodyLPL 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lipoprotein Lipase (LPL) (AA 55-316) antibodyLPL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lipoprotein Lipase (LPL) antibodyLPL 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lipoprotein Lipase (LPL) (AA 162-246) antibodyLPL 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lipoprotein Lipase (LPL) antibody (HRP)LPL 适用: 人, 大鼠, Cow, 小鸡, 豚鼠 WB, IP, ELISA 宿主: 小鼠 Monoclonal 5D2 HRP
anti-Lipoprotein Lipase (LPL) (AA 1-475) antibodyLPL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Lipoprotein Lipase (LPL) antibodyLPL 适用: 人, 大鼠, 小鼠, Golden Syrian Hamster, Cat WB, ELISA, FACS, IF, InhA 宿主: 小鼠 Monoclonal 5D2 unconjugated
anti-Lipoprotein Lipase (LPL) antibodyLPL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-Lipoprotein Lipase (LPL) (AA 300-327) antibodyLPL 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998, PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997, PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000, PMID: 11031206). Immunofluorescence was performed on LPL expressed on CHO-K1 cells using this antibody (Voss et al., 2011, PMID: 21518912). The binding affinity of the antibody to a synthetic LPL peptide containing the Trp-rich loop of human, mouse, bovine, rat and chicken, was measured by surface plasmon resonance.The binding affinity of the antibody to the human, bovine and chicken LPL peptides was high (KD of 0.19, 0.78 nM and 0.34 nM, respectively), the binding affinity for the rat LPL peptide was reduced (Kd= 2.99nM), and no binding to the Trp-rich peptide from mouse LPL was detected. The antibody detected LPL by western blot analysis. By immunocytochemistry, the antibody bound avidly to the wildtype human LPL on CHO cells. The crystal structure of the Fab version of the antibody was solved (Luz et al., 2020, PMID: 32690595).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Lipoprotein Lipase (LPL)
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别名
- Lipoprotein lipase
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背景
- LPL, Phospholipase A1, EC 3.1.1.34
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UniProt
- P06858
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途径
- Lipid Metabolism
抗原
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