Recombinant Glypican 3 抗体 (Extracellular Domain)
Quick Overview for Recombinant Glypican 3 抗体 (Extracellular Domain) (ABIN7543338)
抗原
See all Glypican 3 (GPC3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Anti-Glypican 3 [7H1], Rabbit IgG, kappa
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特异性
- This antibody binds an epitope between amino acids 25 to 137 of the human glypican 3 (GPC3). This antibody cross reacts with cynomolgus monkey, rat and mouse GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC).
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交叉反应
- Cynomolgus, 小鼠, 大鼠
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).
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亚型
- IgG kappa
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anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 9C2 unconjugated
anti-Glypican 3 (GPC3) (AA 344-559) antibodyGPC3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glypican 3 (GPC3) antibodyGPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal GPC3-863 unconjugated
anti-Glypican 3 (GPC3) (AA 55-200) antibodyGPC3 适用: 人 IF, FACS, WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 9C2 unconjugated
anti-Glypican 3 (GPC3) (AA 359-554) antibodyGPC3 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2C7E9 unconjugated
anti-Glypican 3 (GPC3) (AA 55-200) antibodyGPC3 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2A2C3 unconjugated
anti-Glypican 3 (GPC3) antibodyGPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal rGPC3-863 unconjugated Recombinant Antibody
anti-Glypican 3 (GPC3) (AA 511-580) antibodyGPC3 适用: 人 IF, FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal 1G12 unconjugated
anti-Glypican 3 (GPC3) (AA 359-554) antibodyGPC3 适用: 人, 大鼠 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4D5B1 unconjugated
anti-Glypican 3 (GPC3) antibodyGPC3 适用: 人, 小鼠 IF, WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 9C2 unconjugated
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应用备注
- This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. This antibody strongly reacts with hepatic cancer tissue microarray, JHH cells, HepG2 cells, and cells stably transfected with GPC3 in immunohistochemistry. This antibody also detects human, cynomolgus monkey, rat, and mouse GPC3 by western blot. This antibody was also used in the generation of an antibody drug conjugate with maleimide acetal PNU-159682 or monomethyl disulfide N10-linked PBD. The ADCC activity of the antibody drug conjugate was investigated using a human HepG2 X1 and JHH7 xenograft model and substantial tumor growth inhibition was achieved with 7H1-disulfide-PBD (US9926377).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Glypican 3 (GPC3)
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别名
- Glypican 3
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背景
- GPC3, Glypican-3, GPC-3, GTR2-2, MXR7, OCI5, DGSX, Intestinal protein OCI-5
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UniProt
- P51654
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途径
- Glycosaminoglycan Metabolic Process
抗原
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