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Recombinant HSP70 抗体 (AA 450-463)

Name: Recombinant HSP70 抗体 (AA 450-463)
Brand: antibodies-online
SKU: ABIN7543335
Availability: OutOfStock

This anti-HSP70 antibody is a 小鼠单克隆 antibody detecting HSP70 in WB, IHC, ELISA, IF 和 FACS. Suitable for 人.

产品编号 ABIN7543335

发货至: 中国

Quick Overview for Recombinant HSP70 抗体 (AA 450-463) (ABIN7543335)

抗原

See all HSP70 抗体

HSP70 (Heat Shock Protein 70 (HSP70))

抗体类型

Recombinant Antibody

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This HSP70 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

Cmhsp70-1

Discover our top product HSP70 Primary Antibody

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1
AA 450-463

原理

Anti-HSP70 [cmhsp70.1 (C92F3B1)], Mouse IgG1, lambda

特异性

This antibody binds a region including amino acids 473-504 (TKDNNLLGRFELSG) of the C-terminal substrate-binding domain (SBD) of membrane bound Hsp70 (mHsp70). It binds a 14-mer peptide termed"". This protein is located intracellularly and plays an important part in various cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. It plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. It maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Membrane bound Hsp70s is frequently over-expressed in patients affected by leukemia and has been identified as a potential target for cancer therapies.

产品特性

Original Species of Ab: Mouse
Original Format of Ab: IgG1

纯化方法

Protein A affinity purified

免疫原

The original antibody was generated by grafting the CDRs of the mouse antibody on human framework regions. The parental mouse antibody was generated by immunizing mice with an SBD-derived synthetic peptide TKDNNLLGRFELSG (TKD peptide, aa 450-463) with complete Freud's adjuvants.

亚型

IgG1 lambda
anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, ICC, FACS, IEM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody (HRP)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal HRP

anti-Heat Shock Protein 70 (HSP70) antibody (FITC)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal FITC

anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Biotin

anti-Heat Shock Protein 70 (HSP70) antibody (PE)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PE

anti-Heat Shock Protein 70 (HSP70) antibody (APC)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal APC

anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PerCP

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 594)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 594

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 488

应用备注

The IgG1 version of this antibody binds the C-terminal substrate-binding domain (SBD) of the human membrane heat shock protein mHsp70 with an affinity of 3nM. The binding characterization of this antibody was done using ELISA. This antibody can detect aa 473-504) fused to domain III of HSA in a western blot assay (PMID: 20556545). The Fab version of this antibody binds mHsp70 with an affinity of 35 nM. The fab version can successfully identify membrane-associated Hsp70 on tumor cell lines both in immunofluorescence staining and flow cytometry (PMID: 20123884). The original mouse antibody cmHsp70.1 can selectively induce antibody-dependent cellular cytotoxicity (ADCC) of membrane Hsp70+ mouse tumor cells by unstimulated mouse spleen cells. Tumor killing could be further enhanced by activating the effector cells with TKD and IL-2 (PMID: 21187371). Cy5.5-conjugated cmHsp70.1 was capable of being endocytosed and accumulated in the tumor and was used for near-infrared fluorescence imaging of tumors. The tumor-selective internalization of mAb cmHsp70.1 might enable targeted uptake of toxins or radionuclides into Hsp70 membrane-positive tumors (PMID: 20406322). cmHsp70.1 antibody-conjugated gold nanoparticles were used in the real time imaging of small tumors (PMID: 26392771). This antibody was also used in the immunohistochemical and flow cytometric analysis of mHsp70 expressed on glioblastoma multiforme tumors (PMID: 29177668).

限制

仅限研究用
浓度

1 mg/mL

缓冲液

PBS with 0.02 % Proclin 300.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
抗原

HSP70 (Heat Shock Protein 70 (HSP70))

别名

HSP70

背景

HSP70-1, HSP70.1, HSPA1A, HSX70, HSP72, mHsp70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1

UniProt

P0DMV8