Recombinant CD147 抗体
Quick Overview for Recombinant CD147 抗体 (ABIN7543310)
抗原
See all CD147 (BSG) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD147 [5F6], Rabbit IgG, kappa
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特异性
- The antibody binds the N domain of CD147. The antibody binds to an epitope on the extracellular domain of CD147 (residues Asp65-Phe74 and Val30-Thr40). CD147 is a type I membrane glycoprotein and is classified into the immunoglobulin-like superfamily, and is expressed in many types of cancer. It is suggested that CD147 is also associated with cancer metastasis, invasion, and chemical drug resistance.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing mice with CD147.
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亚型
- IgG kappa
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anti-Basigin (Ok Blood Group) (BSG) (AA 79-280) antibody
BSG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 87-323) antibodyBSG 适用: 小鼠 WB, FACS, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 59-321) antibodyBSG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 30-200) antibodyBSG 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7H4D4 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 2D8C11 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 WB, FACS, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3E10C1 unconjugated
anti-Basigin (Ok Blood Group) (BSG) antibodyBSG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal BSG-963 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 1-269) antibodyBSG 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- The specificity of the antibody was coonfirmed by competition ELISA. The antibody recognized MDA-MB-231 cells that express native CD147 antigens on tumor cell surface. To assess the ability of the original antibody to block MMP-1 production, NHLF were treated with recombinant a ECD-Flag construct and the MMP-1 production in response to CD147 stimulation was determined using the MMP-1 activity assay. The antibody showed significant inhibition of MMP-1 production. The original antibody was screened for the ability to inhibit CD147-induced MMP-2 or MMP-9 production in co-culture. Results showed the antibody inhibited MMP-2 and MMP-9 production in co-culture. The humanized version of the antibody (IgG1) inhibited CD147-stimulated MMP-1 production by fibroblast in a dose-dependent manner and the MMP-2 production by MDA-MB-231 and NHDF in a dose dependent manner. The humanized version of the antibody was able to inhibit VEGF production by normal human lung fibroblasts (NHLF) stimulated with 10 μg/mL recombinant CD147 for 48 hours. The humanized version of the antibody inhibited more than 40 % of VEGF production in co-culture of tumor cells and fibroblast cells. The humanized version of the antibody inhibited angiogenesis in vivo. SCID Beige Mice implanted with MDA-MB-231 human breast carcinoma cells were treated with E+5F6(MuIgG2a), the antibody inhibited tumor angiogenesis in Panc-1 matrigel plug model. The antibody showed potential as therapeutic and prophylactic agents (US8618264B2). The structure of the Fab fragment was determined (Teplyakov et al., 2010).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
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别名
- CD147
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背景
- Basigin, Emmprin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, HAb18G, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF,BSG, Cluster of differentiation 147
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UniProt
- P35613
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途径
- S100 Proteins
抗原
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