Recombinant XCR1 抗体
Quick Overview for Recombinant XCR1 抗体 (ABIN7543271)
抗原
See all XCR1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-XCR1 [5G7], Rabbit IgG, kappa
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特异性
- This antibody binds human XCR1, more particularly it strongly binds amino acids 'PESTTFFYYDLQ' of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphotactin. It transduces a signal by increasing the intracellular calcium ions level.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing XCR1 knockout mice with human XCR1-expressing B300.19 cells mixed with equal volume of GERBU adjuvant.
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亚型
- IgG kappa
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anti-Chemokine (C Motif) Receptor 1 (XCR1) (AA 244-322) antibody
XCR1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Extracellular Domain) antibodyXCR1 适用: 人 IHC, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Cytoplasmic Domain) antibodyXCR1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Internal Region) antibodyXCR1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (AA 305-333) antibodyXCR1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 μg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))
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别名
- XCR1
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背景
- CCXCR1, GPR5, chemokine XC receptor 1, G-protein coupled receptor 5, lymphotactin receptor
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UniProt
- P46094
抗原
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