Recombinant XCR1 抗体
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北京 101111
Quick Overview for Recombinant XCR1 抗体 (ABIN7543270)
抗原
See all XCR1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-XCR1 [MARX10], Mouse IgG2b, kappa
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特异性
- This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.
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交叉反应
- 大鼠
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 x 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.
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亚型
- IgG2b kappa
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anti-Chemokine (C Motif) Receptor 1 (XCR1) (AA 244-322) antibody
XCR1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Extracellular Domain) antibodyXCR1 适用: 人 IHC, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (Cytoplasmic Domain) antibodyXCR1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C Motif) Receptor 1 (XCR1) (AA 305-333) antibodyXCR1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20 % of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012, PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1+ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay, MARX10-OVA was found to be highly effective in inducing CD8+ T cell cytotoxicity in vivo (Hartung et al., 2015, PMID: 25520399).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))
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别名
- XCR1
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背景
- MXCR1, Ccxcr1, Chemokine XC receptor 1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1, MARX1O
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UniProt
- Q9R0M1
抗原
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