Recombinant CD47 抗体 (Extracellular Domain)
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Quick Overview for Recombinant CD47 抗体 (Extracellular Domain) (ABIN7543264)
抗原
See all CD47 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Anti-CD47 [ZF1], Rabbit IgG, lambda
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特异性
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This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Application Notes original Clone: This antibody displayed high specificity and affinity for CD47 protein, which were comparable to those for humanized anti-CD47 blocking antibody B6H12. This antibody is could efficiently block the physical interaction of immobilized recombinant human CD47 to human and mouse SIRPα in blocking assay in vitro and was capable of inducing in vitro macrophage-mediated phagocytosis as robust as B6H12. It binds recombinant human CD47 in a dose dependent manner in an ELISA. This antibody was also capable of recognizing cell-surface expressed CD47 in a flow cytometry. The binding affinity of the IgG version of this antibody toward CD47 was determined to be 3.50 ± 0.16 nM using surface plasmon resonance. ZF1 treatment could induce robust phagocytosis of leukemic cancer cells by macrophage in vitro, and protect BALB/c nude mice from cancer killing by engrafted leukemic cells (CCRF and U937) to a similar extent as humanized B6H12 did (PMID: 27863402).
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was isolated after screening of three lambda3-H3, lambda3-H5 and lambda1-H3 phage display single-chain variable fragments (scFvs) libraries by panning against recombinant protein antigen CD47-His. The variable domain genes of the antibody were cloned into a pair of eukaryotic cell expression vectors and co-transfected into FreeStyle HEK293T cells for expression and obtaining the IgG antibody.
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亚型
- IgG, lambda
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anti-CD47 (CD47) antibody
CD47 适用: 人 FACS, WB, IF, Func 宿主: 小鼠 Monoclonal B6H12-2 unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 FACS, IHC, IF, ELISA 宿主: 小鼠 Monoclonal CD47-3019 unconjugated
anti-CD47 (CD47) (AA 20-140) antibodyCD47 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 241-323) antibodyCD47 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal CD47-2937 unconjugated
anti-CD47 (CD47) antibody (FITC)CD47 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-122 FITC
anti-CD47 (CD47) (AA 18-219) antibodyCD47 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人, Pig, Non-Human Primate FACS, WB, IHC (fro) 宿主: 小鼠 Monoclonal MEM-122 unconjugated
anti-CD47 (CD47) (AA 19-142) antibodyCD47 适用: 人 WB, IHC, ICC 宿主: 兔 Monoclonal BLR131H unconjugated Recombinant Antibody
anti-CD47 (CD47) antibodyCD47 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 4G11 unconjugated Recombinant Antibody
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Human
OriginalFormat: IgG1
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD47
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别名
- CD47
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背景
- IAP, MER6, OV-3, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, ovarian cancer marker
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UniProt
- Q08722
抗原
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