Recombinant Liver Arginase 抗体
Quick Overview for Recombinant Liver Arginase 抗体 (ABIN7543253)
抗原
See all Liver Arginase (ARG1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Arginase 1 [mAb1], Mouse IgG2a, kappa
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特异性
- The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80 % of all complexes identified were composed of two arginase 1 trimers and three antibodies, forming a 2:3 complex, while the remaining 20 % of complexes were composed of two arginase 1 trimers and two antibodies, however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The antibody is a human antibody identified via yeast display technologies and isolated after panning against arginase 1.
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亚型
- IgG2a kappa
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anti-Arginase, Liver (ARG1) antibody
KO Validated ARG1 适用: 人 WB, ELISA, FACS, IHC (p), IF, IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (C-Term) antibodyVerified ARG1 适用: 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (C-Term) antibodyVerified ARG1 适用: 人, 大鼠, 小鼠, Pig WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (AA 11-97) antibodyARG1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal ARG1-1125 unconjugated
anti-Arginase, Liver (ARG1) (C-Term) antibodyARG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (N-Term) antibodyARG1 适用: 人, 大鼠, 小鼠, Cow, 兔, 犬, Pig, 马, 山羊, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (AA 293-322) antibodyARG1 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (AA 151-220) antibodyARG1 适用: 人, 大鼠 ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (AA 1-322) antibodyARG1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Arginase, Liver (ARG1) (N-Term) antibodyARG1 适用: 人, 大鼠, 小鼠, Cow, 兔, 犬, Pig, 马, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of human arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM (Palte et al., 2021, PMID: 34326456).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
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别名
- Arginase 1
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背景
- ARG1, Arginase-1, Liver-type arginase, Type I arginase
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UniProt
- P05089
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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