Recombinant LDLR 抗体
Quick Overview for Recombinant LDLR 抗体 (ABIN7543252)
抗原
See all LDLR 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-LDL Receptor [C7], Mouse IgG2b, kappa
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特异性
- The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis.
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交叉反应
- Cow
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was produced by immunization of BALB/C mice with the extra cellular domain of the bovine LDL-R.
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亚型
- IgG2b kappa
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anti-Low Density Lipoprotein Receptor (LDLR) (C-Term) antibody
KO Validated LDLR 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 22-150) antibodyLDLR 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1B10H10 unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 781-860) antibodyLDLR 适用: 人, 小鼠, 大鼠, Hamster, 植物 WB, ELISA, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) antibodyLDLR 适用: 人 IHC, ELISA, FACS, IF 宿主: 兔 Monoclonal 2B10 unconjugated Recombinant Antibody
anti-Low Density Lipoprotein Receptor (LDLR) (AA 497-742) antibodyLDLR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 263-509) antibodyLDLR 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 105-205) antibodyLDLR 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5E7 unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 335-588) antibodyLDLR 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 499-511) antibodyLDLR 适用: 人, 小鼠 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Low Density Lipoprotein Receptor (LDLR) (AA 391-419) antibodyLDLR 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
- The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast, complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981, PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982, PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987, PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001, PMID: 11781697) (Ranheim et al., 2006, PMID: 16740646). Immunofluorescence was performed on liver Chang cells using this antibody, showing that the WT protein was localised on the cell surface whereas the two mutant proteins were localised intracellularly (Holst et al., 2001, PMID: 11781697). The antibody was used to inhibit the binding of LDL to its receptor LDLR in brain capillary endothelial cells. Coincubations of LDL with increasing concentrations of the antibody decreased the rate of passage of the LDL through the monolayer, LDL transcytosis was totally abolished at the concentration of 1 mg/mL of antibody. This confirmed that the LDL receptor is involved receptor-mediated transcytosis (Dehouck et al., 1997, PMID: 9265653).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- LDLR (Low Density Lipoprotein Receptor (LDLR))
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别名
- LDL Receptor
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背景
- LDLR, Low-density lipoprotein receptor, IgG-C7
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UniProt
- P01130
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途径
- Hepatitis C, Lipid Metabolism
抗原
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