Recombinant Fibrin beta-Chain (N-Term) 抗体
适用: 人
ELISA
宿主: 小鼠
Monoclonal
59D8
unconjugated
Recombinant Antibody
-
- 抗原
- Fibrin beta-Chain
- 抗体类型
- Recombinant Antibody
- 抗原表位
- N-Term
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- ELISA
- 原理
- Anti-Fibrin beta-chain [59D8], Mouse IgG1, kappa
- 特异性
- This antibody selectively binds to the amino-terminus of the fibrin β-chain that becomes exposed after cleavage of fibrinopeptide B by thrombin.
- 产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG1
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by using a synthetic heptapeptide from the amino terminus of the beta chain in human fibrin as an immunogen.
- 克隆位点
- 59D8
- 亚型
- IgG1 kappa
-
-
- 应用备注
- The original mouse antibody was able to bind fibrin even in the presence of human fibrinogen at the concentration found in plasma. This antibody when intravenously administered in a chicken ex vivo circulatory model bound human fibrin-coated disks placed in an extracorporeal chamber (PMID: 6648524). An immunoconjugate (tPA-59D8) generated from tissue plasminogen activator (tPA) and anti-fibrin antibody 59D8 degraded less fibrinogen and consumed less alpha 2-antiplasmin than did tPA alone, suggesting that more potent and more selective thrombolysis can be achieved by efficiently directing tPA to the site of a thrombus (PMID: 3118374). This antibody was used in the generation of a single-chain urokinase plasminogen activator (scu-PA) and -59D8 Fab' conjugate which had a fibrinolytic potency 33-fold more than that of tissue plasminogen activator (PMID: 2111744). A recombinant r-scuPA(32 kDa)-59D8 was 6 times more potent than high molecular mass scuPA in lysing a human plasma clot in vitro and was 20 times more potent than high molecular mass scuPA in the rabbit jugular vein model of thrombolysis. The binding affinity of the antibody towards fibrin was determined using ELISA (PMID: 1946453). This antibody was also used in the generation of a fusion protein comprising hirudin that genetically fused to the C-terminus of the variable region of the light chain of scFv59D8. This fusion protein was found to inhibit thrombin after its activation by factor Xa and also inhibits appositional clot growth in vitro (PMID: 10715263).
- 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
- 抗原
- Fibrin beta-Chain
- 背景
- FGB, factor Ia, factor-1a, fibrin, fibrin β chain, fibrin beta chain, Fibrinogen beta chain, Fibrinopeptide B, Fibrinogen beta chain
- UniProt
- P02675
-
You are here:
