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Recombinant CD1d 抗体

This anti-CD1d antibody is a 大鼠 单克隆 antibody detecting CD1d in FACS, IHC, ELISA 和 IF. Suitable for 小鼠.
产品编号 ABIN7543210
发货至: 中国

Quick Overview for Recombinant CD1d 抗体 (ABIN7543210)

抗原

See all CD1d (CD1D) 抗体
CD1d (CD1D) (CD1d Molecule (CD1D))

抗体类型

Recombinant Antibody

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This CD1d antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

克隆位点

1B1
  • 原理

    Anti-CD1d [1B1], Rat IgG2b, kappa

    特异性

    This antibody is specific for CD1d. Its binding is blocked by antibody clone 3D11. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

    产品特性

    Original Species of Ab: Rat

    Original Format of Ab: IgG2b

    纯化方法

    Protein A affinity purified

    免疫原

    The original version of this antibody was raised by immunizing Lewis rats using mCD1-transfected RMA-S cells.

    亚型

    IgG2b kappa
  • 应用备注

    In an attempt to further the understanding of CD1 proteins, flow cytometry was preformed on mouse spleen cells using the rat version of this antibody. Further, mice infected with L. monocytogenes were treated with the rat version of this antibody. Finally, mice spleen cells were stained with the mouse version of this antibody (Szalay et al, 1999, pmid:10358132). To investigate the tissue distribution of murine CD1 in mice, immunohistochemistry was preformed on thymus, small intestine, and spleen tissue of mice using the rat version of this antibody. Further, flow cytometry was preformed on epithelial cells and IEL using the rat version of this antibody (Brassay et al, 1997, pmid:9233616). In order to measure mCD1-restricted T cell responses to glycolipids, the rat version of this antibody was added after which cytokine levels were assessed by ELISA (Burdin et al, 1998, pmid:9759842). To assess the role of CD1 in the pathogenesis of lupus the rat version of this antibody was used for immunofluorescent staining and flow cytometry on single cell suspensions of mouse spleen cells or mouse bone marrow. The mouse version of this antibody was further tested in vivo on mice with lupus. Their lifespan showed an increase (Zeng et al, 2000, pmid:10799851). To study the expression of CD1 on B cell subsets from normal and b2m2/2 mice, single-cell suspensions of lymphoid cells from C57BL/6 and BALB/c mice were used for immunofluorescence using the mouse version of this antibody (Amano et al, 1998, pmid:9712035). To eludicate the location of expression of CD1 flow cytometry was preformed on thymocytes from wild-type, b2m-deficient, and TAP-1-deficient C57BL/6 mice (Roark et al, 1998, pmid:9531266).

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    CD1d (CD1D) (CD1d Molecule (CD1D))

    别名

    CD1d

    背景

    Antigen-presenting glycoprotein CD1d1, Cd1d1, CD1d.1

    UniProt

    P11609

    途径

    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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