Recombinant CD1d 抗体
Quick Overview for Recombinant CD1d 抗体 (ABIN7543209)
抗原
See all CD1d (CD1D) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD1d [1B1], Rabbit IgG, kappa
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特异性
- This antibody is specific for CD1d. Its binding is blocked by antibody clone 3D11. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- The original version of this antibody was raised by immunizing Lewis rats using mCD1-transfected RMA-S cells.
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亚型
- IgG kappa
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anti-CD1d Molecule (CD1D) (AA 56-271) antibody
CD1D 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) (AA 32-302) antibodyCD1D 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) antibodyCD1D 适用: 小鼠, 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) antibody (PE)CD1D 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 51-1 PE
anti-CD1d Molecule (CD1D) (AA 20-190) antibodyCD1D 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) antibodyCD1D 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) (AA 1-335) antibodyCD1D 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) (Internal Region) antibodyCD1D 适用: 小鼠, 人, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD1d Molecule (CD1D) antibodyCD1D 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 51-1 unconjugated
anti-CD1d Molecule (CD1D) (AA 64-298) antibodyCD1D 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- In an attempt to further the understanding of CD1 proteins, flow cytometry was preformed on mouse spleen cells using the rat version of this antibody. Further, mice infected with L. monocytogenes were treated with the rat version of this antibody. Finally, mice spleen cells were stained with the mouse version of this antibody (Szalay et al, 1999, pmid:10358132). To investigate the tissue distribution of murine CD1 in mice, immunohistochemistry was preformed on thymus, small intestine, and spleen tissue of mice using the rat version of this antibody. Further, flow cytometry was preformed on epithelial cells and IEL using the rat version of this antibody (Brassay et al, 1997, pmid:9233616). In order to measure mCD1-restricted T cell responses to glycolipids, the rat version of this antibody was added after which cytokine levels were assessed by ELISA (Burdin et al, 1998, pmid:9759842). To assess the role of CD1 in the pathogenesis of lupus the rat version of this antibody was used for immunofluorescent staining and flow cytometry on single cell suspensions of mouse spleen cells or mouse bone marrow. The mouse version of this antibody was further tested in vivo on mice with lupus. Their lifespan showed an increase (Zeng et al, 2000, pmid:10799851). To study the expression of CD1 on B cell subsets from normal and b2m2/2 mice, single-cell suspensions of lymphoid cells from C57BL/6 and BALB/c mice were used for immunofluorescence using the mouse version of this antibody (Amano et al, 1998, pmid:9712035). To eludicate the location of expression of CD1 flow cytometry was preformed on thymocytes from wild-type, b2m-deficient, and TAP-1-deficient C57BL/6 mice (Roark et al, 1998, pmid:9531266).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD1d (CD1D) (CD1d Molecule (CD1D))
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别名
- CD1d
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背景
- Antigen-presenting glycoprotein CD1d1, Cd1d1, CD1d.1
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UniProt
- P11609
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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